Plant Transcription Factor Database
Previous version: v3.0
Citrullus lanatus
HSF Family
Species TF ID Description
Cla000713HSF family protein
Cla002208HSF family protein
Cla002516HSF family protein
Cla002923HSF family protein
Cla006031HSF family protein
Cla006194HSF family protein
Cla007345HSF family protein
Cla008825HSF family protein
Cla009616HSF family protein
Cla009714HSF family protein
Cla010632HSF family protein
Cla011478HSF family protein
Cla013502HSF family protein
Cla014794HSF family protein
Cla016209HSF family protein
Cla016764HSF family protein
Cla016837HSF family protein
Cla017729HSF family protein
Cla019642HSF family protein
Cla020657HSF family protein
Cla021395HSF family protein
Cla021592HSF family protein
Cla022583HSF family protein
Cla022683HSF family protein
HSF Family Introduction

Heat stress transcription factors (Hsfs) are the major regulators of the plant heat stress (hs) response. Sequencing of the Arabidopsis genome revealed the existence of 21 open-reading frames (ORFs) encoding putative Hsfs assigned to classes A-C. Here we present results of a functional genomics approach to the Arabidopsis Hsf family focused on the analysis of their C-terminal domains (CTDs) harboring conserved modules for their function as transcription factors and their intracellular localization. Using reporter assays in tobacco protoplasts and yeast as well as glutathione-S-transferase (GST) pull-down assays, we demonstrate that short peptide motifs enriched with aromatic and large hydrophobic amino acid (aa) residues embedded in an acidic surrounding (AHA motifs) are essential for transcriptional activity of class A Hsfs. In contrast to this, class B and C Hsfs lack AHA motifs and have no activator function on their own. We also provide evidence for the function of a leucine (Leu)-rich region centered around a conserved QMGΦL motif at the very C-terminus as a nuclear export signal (NES) of class A Hsfs. Sequence comparison indicates that the combination of a C-terminal AHA motif with the consensus sequence FWxxF/L,F/I/L as well as the adjacent NES represents a signature domain for plant class A Hsfs, which allowed to identify more than 60 new Hsfs from the expressed sequence tag (EST) database.

Kotak S, Port M, Ganguli A, Bicker F, von Koskull-Doring P.
Characterization of C-terminal domains of Arabidopsis heat stress transcription factors (Hsfs) and identification of a new signature combination of plant class A Hsfs with AHA and NES motifs essential for activator function and intracellular localization.
Plant J, 2004. 39(1): p. 98-112.
PMID: 15200645